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In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

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De Novo Designed Protein-Interaction Modules for In-Cell Applications.

Caitlin L Edgell^1,2, Abigail J Smith^2,3, Joseph L Beesley¹

¹School of Chemistry , University of Bristol , Bristol , BS8 1TS , United Kingdom.

ACS Synthetic Biology

|January 25, 2020

Summary

Newly designed protein coils mediate interactions in bacteria. These synthetic coiled coils offer versatile control for protein assembly in synthetic biology applications, enabling precise biological process engineering.

Keywords:

Lac repressor artificial transcription factor coiled coil de novo protein design transcriptional control

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Published on: July 8, 2025

Area of Science:

Synthetic biology
Protein engineering
Biophysics

Background:

Protein-protein interactions are crucial for biological processes.
Alpha-helical coiled coils are common mediators of these interactions.
De novo design offers control over protein interaction properties.

Purpose of the Study:

To test de novo designed coiled coils for mediating protein-protein interactions in Escherichia coli.
To evaluate the in vivo functionality of designed coiled coil modules.
To demonstrate the versatility and controllability of designed protein interaction domains.

Main Methods:

Design and synthesis of various coiled coil structures (homodimer, homotetramer, heterotetramer).
In vitro characterization using biophysical and structural methods.
In vivo testing using a transcription repression assay in Escherichia coli.

Main Results:

Designed coiled coils functioned as intended within the cellular environment.
Different coiled coil designs conferred distinct properties to Lac repressor complexes.
Assembly and disruption of protein complexes could be controlled by component expression and helix sequestration.

Conclusions:

De novo designed coiled coils are effective protein-protein interaction domains in vivo.
These versatile modules enable reconfigurable and controllable protein assembly for synthetic biology.
The simplicity of the designs allows for future modifications and broader applications.