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Published on: May 21, 2019
Redox-Active Bis-Cyclometalated Iridium(III) Complex as a DNA Photo-Cleaving Agent
Justin Weynand1,2, Angélica Moreno-Betancourt2, Frédérique Loiseau2
1Institut de la Matière Condensée et des Nanosciences (IMCN), Molecular Chemistry, Materials and Catalysis Division (MOST) , Université catholique de Louvain (UCLouvain) , Place Louis Pasteur 1, bte L4.01.02 , B-1348 Louvain-la-Neuve , Belgium.
Abstract:
The development of new photoactive metal complexes that can trigger oxidative damages to the genetic material is of great interest. In the present paper, we describe the detailed study of a highly photo-oxidant iridium(III) complex that triggers photoinduced electron transfer (PET) with purine DNA bases. The PET has been studied by luminescence and laser flash photolysis experiments. From plasmid DNA agarose gel electrophoresis experiments, we demonstrated the high ability of the iridium complex to induce strand breaks upon light irradiation. Reactive oxygen species (ROS)-specific scavengers and stabilizers were employed to identify that the photocleavage process, the results of which infer singlet oxygen and hydrogen peroxide as the predominant species. To the best of our knowledge, the present work represents one of the few study for highly photo-oxidant bis-cyclometalated iridium(III) complex toward DNA.
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