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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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KPC-producing Enterobacterales with uncommon carbapenem susceptibility profile in Vitek 2 system

Ana Paula Cury1, Raquel Girardello2, Alberto José da Silva Duarte3

  • 1Microbiology Laboratory, Central Laboratory Division-LIM 03, Pathology Department, Hospital das Clínicas, Universidade de São Paulo, Brazil.

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
|January 25, 2020
PubMed

Insights

Carbapenem-resistant Klebsiella pneumoniae poses a significant challenge. Certain carbapenem-resistant Enterobacterales strains exhibited unusual susceptibility patterns, showing resistance to some carbapenems but susceptibility via alternative testing methods.

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Antimicrobial resistance

Background:

  • KPC-producing Klebsiella pneumoniae is a major global health threat.
  • Endemic carbapenem-resistant Enterobacterales (CRE) strains present diagnostic challenges.

Purpose of the Study:

  • To investigate an unusual carbapenem susceptibility profile in KPC-producing Enterobacterales.
  • To evaluate discrepancies in carbapenem testing methods for CRE.

Main Methods:

  • Seven KPC-producing Enterobacterales isolates were analyzed.
  • Carbapenem susceptibility was tested using Vitek 2™, E-test™, and disk diffusion methods.
  • Minimum Inhibitory Concentrations (MICs) for ertapenem, imipenem, and meropenem were determined.

Main Results:

  • All seven strains were resistant to at least one carbapenem.
  • Vitek 2™ system showed ertapenem MICs lower than imipenem/meropenem MICs.
  • E-test™ and disk diffusion methods indicated susceptibility to carbapenems.

Conclusions:

  • Discrepancies in carbapenem susceptibility testing were observed for KPC-producing Enterobacterales.
  • Standardized testing protocols are crucial for accurate detection of carbapenem resistance.
  • Further investigation into these unusual resistance patterns is warranted.
Keywords:
CarbapenemasesCarbapenemsEnterobacteralesKPCVitek 2™ system

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