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An improved standardized procedure for urine mutagenicity testing.

A Rannug1, M Olsson, L Aringer

  • 1Department of Toxicology, National Institute of Occupational Health, Solna, Sweden.

IARC Scientific Publications
|January 1, 1988
PubMed
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Optimized urinary mutagenicity testing reduces background variation and enhances sensitivity. This improved method significantly increases mutagen recovery from smokers' urine, aiding in environmental exposure assessments.

Area of Science:

  • Environmental Health
  • Toxicology
  • Analytical Chemistry

Background:

  • Urinary mutagenicity testing is crucial for assessing exposure to mutagens.
  • Baseline variation and low sensitivity limit current methods.
  • Standardizing diet and using urine from smokers and spiked samples are key.

Purpose of the Study:

  • To optimize urinary mutagenicity testing procedures.
  • To decrease baseline variation in mutagenic activity.
  • To increase the sensitivity of mutagen detection in urine.

Main Methods:

  • Investigated parameters: urine volume, flow rate, pH, ionic strength, and histidine elimination.
  • Utilized XAD-2 and C18 Sep-Pak resins for mutagen recovery.
  • Employed urine from non-smokers, smokers, and chemically spiked samples.

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  • Standardized diet for all subjects.

    • Main Results:

    • Optimized procedure achieved consistent mutagen recovery efficiency for XAD-2 and C18 Sep-Pak resins.
    • The optimized method, using 50 ml acidified urine per cartridge/resin, yielded over tenfold greater mutagen recovery from smokers' urine compared to previous reports.
    • Effective elimination of histidine and reduction in background variation were observed.

    Conclusions:

    • The optimized procedure significantly enhances the sensitivity and reliability of urinary mutagenicity testing.
    • This advancement allows for more accurate assessment of exposure to mutagenic agents.
    • The improved method provides a valuable tool for toxicological and environmental health research.