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A novel immobilization fluorescence capillary analysis method and its applications
Yong-Sheng Li1, Qiao-Jing Li, Xiu-Feng Gao
1School of Chemical Engineering, Sichuan University, Chengdu 6100651, China. lysgxf2005@qq.com.
The Analyst
|January 28, 2020
Summary
Fluorescence capillary analysis (FCA) offers a green, cost-effective method for trace-level sample analysis. This technique uses a capillary for reactions and detection, with broad applications in clinical and food safety testing.
Area of Science:
- Analytical Chemistry
- Green Chemistry
- Biotechnology
Background:
- Fluorescence capillary analysis (FCA) enables trace-level analysis of micro-volume samples.
- FCA is characterized by low cost, ease of operation, and reduced environmental impact, aligning with green analytical chemistry principles.
- It finds applications in clinical, biochemical, pharmaceutical, and food safety fields.
Purpose of the Study:
- To provide a tutorial review of Fluorescence Capillary Analysis (FCA).
- To highlight the principles, components, and diverse applications of FCA.
- To discuss the potential of FCA in advancing analytical technologies and its societal benefits.
Main Methods:
- FCA utilizes a micro-volume glass capillary as a reaction vessel and reagent immobilizer.
- The capillary, modified with functional reagents, samples liquid for reaction and is then placed in a fluorescence detector.
- Methods involve immobilized enzymes, gene probes, or reagents within the capillary for detection.
Main Results:
- Successfully applied FCA for determining various analytes including coenzyme I, ethanol, lactic acid, pyruvic acid, glucose, and sulfated bile acid.
- Demonstrated FCA for serum analysis, urine analysis, cellular analysis, and DNA labeling.
- Showcased FCA's adaptability for simultaneous multi-analyte determination using capillary arrays and mobile health applications.
Conclusions:
- FCA is a versatile and efficient technique for trace-level analysis with significant advantages.
- Its green chemistry characteristics and broad applicability suggest substantial social benefits in medicine, pharmacy, food, and environmental protection.
- Future potential includes advanced capillary array systems and mobile health integration.
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