Quantification of Meloxicam in Human Plasma Using Ionic Liquid-Based Ultrasound-Assisted In Situ Solvent Formation...

Area of Science:

• Analytical Chemistry
• Separation Science
• Pharmacokinetics

Background:

• Meloxicam (MEL) determination in biological matrices requires robust extraction techniques.
• Existing methods may face challenges with sample matrix complexity and ionic strength variations.
• Development of sustainable and efficient extraction methods is crucial for drug analysis.

Purpose of the Study:

• To develop and validate a novel ionic liquid-based ultrasound-assisted in situ solvent formation microextraction (IL-UA-ISFME) coupled with HPLC-UV for meloxicam determination.
• To establish a sustainable and robust analytical method for meloxicam in human plasma.
• To evaluate the method's applicability in pharmacokinetic studies.

Main Methods:

• Ionic liquid-based ultrasound-assisted in situ solvent formation microextraction (IL-UA-ISFME) was employed for sample preparation.
• High-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used for meloxicam quantification.
• Optimization of key microextraction parameters was performed to enhance efficiency.

Main Results:

• The method demonstrated excellent linearity (5-1,500 ng mL-1, R²=0.997) and low limits of detection (1 ng mL-1) and quantification (5 ng mL-1).
• High recovery rates (82.1-93.6%) and good precision (intra-assay: 3.6-4.8%, inter-assay: 3.3-5.1%) were achieved.
• The method proved effective for meloxicam determination in human plasma, yielding pharmacokinetic data.

Conclusions:

• The developed IL-UA-ISFME-HPLC-UV method is a sustainable, accurate, and reliable approach for meloxicam analysis in human plasma.
• The technique offers efficient sample cleanup and is suitable for pharmacokinetic screening.
• This method addresses challenges related to sample ionic strength variations.