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Updated: Dec 30, 2025

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
Published on: November 21, 2013
Assembly into β-Sheet Structures upon Peptide-Liposome Conjugation through Copper(I)-Catalyzed [3+2] Azide-Alkyne
Silvia Cavalli1, Mark Overhand2, Alexander Kros2
1Center for Advanced Biomaterials for Health Care IIT@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125, Naples (Italy), Fax: (+39) 081-1993-3140.
Abstract:
The random-coil-to-β-sheet transition of the (Leu-Glu)4 peptide motif is induced upon its conjugation to the liposome surface through "click" chemistry. Circular dichroism (CD) spectroscopy enables the in situ monitoring of this reaction. A change in the peptide secondary structure is already observed within 15 min. The β-sheet conformation becomes the dominant secondary structure after 45 min, as shown by the strong CD signal observed, which is typical for a β-sheet peptide secondary structure.
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