DNA Fragment Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP)
Mayu Isono1, Satoru Hashimoto2
1Department of Genetics, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusaku, Nagoya, Japan.
Abstract:
Chromatin immunoprecipitation (ChIP) is a versatile method to investigate the interaction between specific proteins and DNA regions in vivo. The success of ChIP experiments is highly dependent on the quality of chromatin preparation and especially DNA fragmentation. To ascertain whether DNA fragmentation is appropriate for ChIP experiments, agarose gel electrophoresis is required. Here we describe the experimental procedure of agarose gel electrophoresis to verify whether DNA fragmentation is appropriate for ChIP-slot blot experiments.
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