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Updated: Dec 29, 2025

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels
Wenmei Zhang1, Lei Liu, Qi Zhang
1Center Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China. xiayanwang@bjut.edu.cn gsguo@bjut.edu.cn.
Abstract:
A highly sensitive laser-induced fluorescence detection system with real-time imaging focusing instead of the use of fluorescent reagents were developed for the detection of analytes in nanocapillaries. A cylindrical lens was adopted for the shaping of a laser beam to increase its spatial resolution. The limit of detection with a capillary having a radius of 440 nm was 6.76 yoctomoles (or four molecules) for fluorescein, and 84.5 yoctomoles (or 51 molecules) for FAM-labeled Hsa-miR-17. The designed system exhibited high sensitivity and stability for femtoliters of sample and was hence indicated to be suitable for analyses of single cells.
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