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Published on: June 23, 2020
A fluorescent naphthalimide NADH mimic for continuous and reversible sensing of cellular redox state
Hemant Sharma1, Nian Kee Tan2, Natalie Trinh2
1School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria 3216, Australia. fred.pfeffer@deakin.edu.au.
Abstract:
A fluorescent, naphthalimide-based, NADH mimic has been synthesised as a reversible, biocompatible, "on-off" probe for the detection of changes in intracellular redox environment (both oxidation and reduction). Interconversion was confirmed by means of electrochemistry and also 1H NMR, UV-vis and fluorescence spectroscopy. The reversibility was also successfully detected in A549 cells under simulated redox stress.
