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3D-printed graphene direct electron transfer enzyme biosensors
Adaris M López Marzo1, Carmen C Mayorga-Martinez1, Martin Pumera2
1Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
Biosensors & Bioelectronics
|January 31, 2020
Summary
Researchers developed a novel enzyme-based biosensor using 3D printing technology. This innovative electrochemical biosensor detects hydrogen peroxide, offering potential for environmental and biomedical applications.
Area of Science:
- Electrochemistry
- Biotechnology
- Materials Science
Background:
- Three-dimensional (3D) printing offers advanced fabrication capabilities for customized electrochemical devices.
- 3D-printed electrodes are emerging as platforms for biosensing, energy storage, and generation.
- Enzyme-based biosensors are crucial for detecting various analytes in environmental and biomedical fields.
Purpose of the Study:
- To fabricate a direct electron transfer enzyme-based biosensor for hydrogen peroxide detection using 3D printing technology.
- To investigate the use of a 3D-printed graphene/polylactic acid (PLA) electrode functionalized with horseradish peroxidase (HRP).
- To explore the role of gold nanoparticles in enhancing electron transfer for improved biosensor performance.
Main Methods:
- Fabrication of a 3D-printed graphene/polylactic acid (PLA) electrode via additive manufacturing.
- Immobilization of horseradish peroxidase (HRP) onto the 3D-printed electrode surface.
- Incorporation of gold nanoparticles to facilitate heterogeneous electron transfer.
- Development of an electrochemical biosensor for hydrogen peroxide detection.
Main Results:
- Successful fabrication of a 3D-printed electrode suitable for biosensor development.
- Demonstration of direct electron transfer from immobilized HRP.
- Enhanced hydrogen peroxide detection facilitated by gold nanoparticles.
- Creation of a third-generation electrochemical biosensor.
Conclusions:
- 3D printing technology provides a versatile platform for fabricating advanced electrochemical biosensors.
- The developed HRP-based biosensor shows promise for sensitive hydrogen peroxide detection.
- This approach has significant implications for developing novel biosensing tools in environmental and biomedical fields.
Keywords:
3D-printed electrodesDirect electron transferGraphene/polylactic acidHorseradish peroxidase
