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A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.

Nuo Duan1, Mofei Shen2, Shuo Qi2

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 31, 2020
PubMed
Summary
This summary is machine-generated.

A new aptamer-based method using surface-enhanced Raman scattering (SERS) detects multiple foodborne pathogens. This sensitive SERS platform accurately identifies pathogens in seafood, offering improved food safety.

Keywords:
AptamerFoodborne pathogensMultiplexPDMSSERSSeafood

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Foodborne pathogens pose significant public health risks.
  • Sensitive and rapid detection methods are crucial for food safety.
  • Current detection methods may lack specificity or speed.

Purpose of the Study:

  • To develop a sensitive aptamer-based method for detecting multiple foodborne pathogens.
  • To utilize surface-enhanced Raman scattering (SERS) technology for enhanced detection.
  • To validate the method's efficacy in real food matrices.

Main Methods:

  • Fabrication of a gold nanoparticle-coated polydimethylsiloxane (Au-PDMS) substrate.
  • Functionalization of the substrate with specific pathogen aptamers (Apt).
  • Development of aptamer-functionalized gold nanoparticle SERS probes with Raman reporters (4-MBA/NBA).
  • Implementation of a sandwich assay for pathogen capture and SERS detection.

Main Results:

  • Selective detection of Vibrio parahaemolyticus at 18 cfu/mL and Salmonella typhimurium at 27 cfu/mL.
  • Successful application in detecting pathogens in seafood samples.
  • Achieved high recovery rates (82.9% to 95.1%) in seafood analysis.
  • Demonstrated the potential for multiplex pathogen detection.

Conclusions:

  • The developed aptamer-SERS method offers high sensitivity and specificity for foodborne pathogen detection.
  • This platform provides a promising tool for rapid and accurate food safety monitoring.
  • The method's successful application in seafood highlights its practical utility.