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A dual-mode electrochemical-colorimetric aptasensor based on HCR-CRISPR/Cas12a cascade amplification for MTGase
Xiaoyao Wang1, Zepeng Gu1, Qinyan Xu1
1School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Food Research International (Ottawa, Ont.)
|July 6, 2026
Summary
A novel dual-mode aptasensor was developed for detecting microbial transglutaminase (MTGase) in food. This highly sensitive method uses a combination of aptamers, cascade amplification, and nanomaterials for reliable trace detection.
Area of Science:
- Food Science
- Analytical Chemistry
- Biotechnology
Background:
- Microbial transglutaminase (MTGase) is extensively used in food processing.
- MTGase can cause immunoreactivity in wheat products, potentially leading to celiac disease and intestinal damage.
- Sensitive detection methods for MTGase are crucial for food safety.
Purpose of the Study:
- To develop a highly sensitive dual-mode aptasensor for the trace detection of MTGase.
- To integrate aptamer recognition, cascade amplification, and bifunctional nanomaterials for enhanced signal output.
- To provide a reliable method for assessing MTGase levels in food matrices.
Main Methods:
- Development of an electrochemical-colorimetric dual-mode aptasensor utilizing HCR-CRISPR/Cas12a cascade amplification.
- Incorporation of MXene@AuNPs for conductivity and Co-Fc-MOF for electrochemical and peroxidase-like activity.
- Utilizing high-affinity aptamers for specific MTGase recognition.
Main Results:
- Achieved a wide linear range of 0.5-2000 ng/mL for MTGase detection.
- Demonstrated low detection limits of 0.12 ng/mL (electrochemical) and 0.14 ng/mL (colorimetric).
- Exhibited excellent stability, reproducibility, and satisfactory recovery in real food sample analysis.
Conclusions:
- The developed dual-mode aptasensor offers a rapid, sensitive, and reliable method for trace MTGase detection in food.
- The integration of aptamers, cascade amplification, and nanomaterials provides a robust sensing platform.
- This approach offers valuable guidance for developing multi-mode aptasensors for food safety applications.
