Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

513
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
513
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

424
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
424

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short-chain fatty acid-producing microbes differentiate non-infectious and infectious neutropenic fever in leukemia.

mSystems·2026
Same author

Gut microbiome features and resistome elements associated with colonization and infection with antibiotic-resistance threats.

Gut microbes reports·2026
Same author

Pangenome Analysis of <i>Proteus mirabilis</i> Reveals Lineage-Specific Antimicrobial Resistance Profiles and Discordant Genotype-Phenotype Correlations.

bioRxiv : the preprint server for biology·2025
Same author

Utilization of machine learning to predict antibiotic resistant event outcomes in acute myeloid leukemia patients undergoing induction chemotherapy.

Frontiers in cellular and infection microbiology·2025
Same author

Characterization of Antibiotic Administration Factors Associated with Microbiome Disruption and Subsequent Antibiotic-Resistant Infection and Colonization Events in Acute Myeloid Leukemia Patients Receiving Chemotherapy.

Antibiotics (Basel, Switzerland)·2025
Same author

<i>Staphylococcus epidermidis</i> in Acute Myeloid Leukemia: A Comparative Genomic Study Against Non-AML Isolates.

Pathogens (Basel, Switzerland)·2025

Related Experiment Video

Updated: Dec 29, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

30.9K

Tools for Analysis of the Microbiome.

Jessica Galloway-Peña1,2,3, Blake Hanson4,5

  • 1Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 1954, Houston, TX, 77030, USA. jrgalloway@mdanderson.org.

Digestive Diseases and Sciences
|February 1, 2020
PubMed
Summary

The human microbiome is crucial for health and disease. This review covers metagenomics methods and highlights challenges for developing microbiome-based therapies.

Keywords:
Data analysisMetagenomicsMicrobiomeVisualization

More Related Videos

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.4K
A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

9.2K

Related Experiment Videos

Last Updated: Dec 29, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

30.9K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.4K
A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

9.2K

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • The human microbiome plays a critical role in health and disease.
  • Metagenomics, using next-generation sequencing, identifies and quantifies microorganisms in human samples.

Purpose of the Study:

  • To review methods, technologies, and tools for microbiome characterization.
  • To discuss considerations for robust microbiome research in health and disease.

Main Methods:

  • Review of current metagenomic technologies and computational tools.
  • Discussion of model systems for microbiome studies.
  • Analysis of challenges in standardization and validation.

Main Results:

  • Metagenomics has advanced significantly but faces limitations.
  • Variability in cohorts, experimental design, and workflows impacts results.
  • Key considerations for robust microbiome investigation are outlined.

Conclusions:

  • Understanding the microbiome is vital for innovative treatments.
  • Addressing computational and standardization challenges is crucial.
  • Robust research is needed before therapeutic interventions.