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Updated: Dec 29, 2025
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
Published on: September 22, 2020
Xenoantibodies and Complement Activity Determinations by Flow Cytometry in Pig-to-Primate Xenotransplantation
Nieves Doménech1,2, Pilar Sánchez-Corral3,4
1Instituto de Investigación Biomédica A Coruña (INIBIC)-Complejo Hospitalario Universitario A Coruña (CHUAC), Coruña, Spain. Nieves.Domenech.Garcia@sergas.es.
Abstract:
In pig-to-primate xenotransplantation, flow cytometry assays allow the examination of antibody reactivity to intact antigens in their natural conformation and location on cell membranes. Here we describe in detail the procedures of two flow cytometry assays to measure the antibody-mediated complement-dependent cytotoxicity (CDC) response or serum levels of IgG and IgM xenoantibodies. This information is key for understanding the rejection process of vascularized xenografts and finding strategies to overcome it.
