Aerobic bacteriological profile and antimicrobial susceptibility pattern of pus isolates from tertiary care hospital
Sukanya Sudhaharan1, Padmaja Kanne2, Padmasri Chavali3
1Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India. sukanyavimala@gmail.com.
Introduction:
Pyogenic infections are an important cause of sepsis. These infections are difficult to treat because of the pathogens with increasing antibiotic resistance. It is important to know the pathogens causing the infections and its antibiotic susceptibility for proper management of the patients.
Methodology:
A retrospective analysis of 1428 culture positive pus and tissue samples received in the department of microbiology from various departments in the hospital between January 2012 to 2017 was performed. Data regarding the pathogen isolated and its antimicrobial susceptibility were collected and analyzed. The specimens were primarily processed, as per standard methods. Identification and susceptibility testing was done using the Vitek-2C system.
Results:
Among the samples males outnumbered females (M: F-2.5:1) and the median age was 47 years. The total number of patients were 1428 with total number of isolates being 1525 as in our study monomicrobial infections were seen in 93.2% (1331/1428) patients whereas combined infections with growth of two pathogens in 6.8% (97/1428). Gram-negative bacilli were isolated in 68.3% (1042/1525). Among the Gram-negative bacilli Escherichia coli was the major pathogen isolated (38.6%, 403/1042). Gram positive organisms were isolated in 31.6% (483/1525) of cases and Staphylococcus aureus was the predominant organism isolated (91.7%, 443/483). Rare pathogens like Burkholderia pseudomallei in 3 patients and Nocardia in one patient were also isolated.
Conclusion:
This study emphasizes to understand the common organisms isolated from wound infections and it helps in empirical treatment of patients based on antibiotic susceptibility patterns.
Insights
This study analyzed 1428 wound infection samples, identifying common pathogens like Escherichia coli and Staphylococcus aureus. Understanding their antibiotic susceptibility is crucial for effective empirical treatment in sepsis management.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Pyogenic infections are a significant cause of sepsis, often complicated by increasing antibiotic resistance.
- Effective patient management necessitates knowledge of causative pathogens and their antibiotic susceptibility profiles.
Purpose of the Study:
- To identify common bacterial pathogens isolated from pus and tissue samples in a hospital setting.
- To analyze the antimicrobial susceptibility patterns of these isolated pathogens.
- To inform empirical treatment strategies for wound infections and sepsis.
Main Methods:
- Retrospective analysis of 1428 culture-positive pus and tissue samples collected between January 2012 and 2017.
- Standard microbiological methods for specimen processing.
- Identification and antimicrobial susceptibility testing performed using the Vitek-2C system.
Main Results:
- Gram-negative bacilli were the predominant isolates (68.3%), with Escherichia coli being the most common (38.6%).
- Gram-positive organisms accounted for 31.6%, with Staphylococcus aureus being the most frequent (91.7%).
- Monomicrobial infections were observed in 93.2% of cases.
Conclusions:
- The study highlights the prevalent pathogens in wound infections, aiding in the selection of appropriate empirical antibiotic therapy.
- Understanding local antibiotic susceptibility patterns is vital for optimizing patient care and combating antimicrobial resistance.
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