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Updated: Dec 29, 2025

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Enhanced enzymatic digestibility of poplar wood by quick hydrothermal treatment
Liping Tan1, Zhongyang Liu2, Tongtong Zhang3
1State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Abstract:
To elevate the glucose yield from the enzymatic hydrolysis of poplar wood for bio-ethanol production, quick hydrothermal treatment (QHT) was conducted at 200 °C for a short period of time from 5 min to 25 min. It was found that the QHT could remove >85% of the hemicelluloses and ~30% of the lignin in the poplar wood, and achieve 82% cellulose conversion at a low cellulase dosage of 10 FPU/g substrate. The enhancement digestibility of poplar wood was ascribed to the higher accessibility of cellulose, as the specific surface area of the substrate increased from 3.0 m2/g to 7.1 m2/g from the of untreated wood to the QHT-treated wood. The results demonstrate the improvements in digestibility and hydrolysis rates after QHT.
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