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High-Throughput Screening Technology in Industrial Biotechnology.

Weizhu Zeng1, Likun Guo2, Sha Xu2

  • 1National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

Trends in Biotechnology
|February 2, 2020
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Summary

High-throughput screening (HTS) strategies enhance industrial microorganisms by improving library diversity and detection methods. Future advancements integrate synthetic biology, nanotechnology, and AI for more efficient microbial applications.

Keywords:
biosensorhigh-throughput screeningmicrofluidicsmutant librarysynthetic biology

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Area of Science:

  • Industrial Biotechnology
  • Microbial Engineering

Background:

  • High-throughput screening (HTS) is crucial for advancing industrial microorganisms.
  • Development of automated devices and rapid assays has enabled sophisticated HTS strategies.

Purpose of the Study:

  • To discuss key factors for improving HTS efficiency in industrial biotechnology.
  • To summarize HTS applications for enhancing industrial microorganism performance.
  • To explore current challenges and future improvements in HTS.

Main Methods:

  • Reviewing significant factors for HTS efficiency, including library construction and detection methods.
  • Summarizing existing applications of HTS in industrial microbiology.
  • Analyzing challenges and potential advancements in HTS.

Main Results:

  • High diversity in screening libraries and novel detection methods are critical for HTS success.
  • HTS has been successfully applied to enhance various industrial microorganisms.
  • Integration of synthetic biology, nanotechnology, and AI offers promising avenues for HTS improvement.

Conclusions:

  • Optimizing HTS strategies is essential for developing superior industrial microorganisms.
  • Future HTS development will be driven by interdisciplinary integration, leading to broader biotechnological applications.