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Related Experiment Videos

Superoxide dismutase from Ascaris suum.

M Sanchez-Moreno1, M Monteoliva, A Fatou

  • 1Instituto López-Neyra de Parasitología, C.S.I.C. Sección de Bioquímica, Granada, Spain.

Parasitology
|October 1, 1988
PubMed
Summary

This study identified and purified three superoxide dismutases (SOD) in Ascaris suum, revealing distinct cellular locations and activities, particularly high levels in female reproductive organs, highlighting SOD's metabolic importance in this parasite.

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Area of Science:

  • Biochemistry
  • Parasitology
  • Enzymology

Background:

  • Superoxide dismutase (SOD) is a crucial antioxidant enzyme.
  • Understanding SOD in helminth parasites like Ascaris suum is important for parasite metabolism and host-pathogen interactions.

Purpose of the Study:

  • To detect, purify, and characterize the different forms of superoxide dismutase (SOD) in Ascaris suum.
  • To investigate the cellular localization and biochemical properties of these SOD forms.
  • To determine the distribution of SOD activity across different tissues of A. suum.

Main Methods:

  • Homogenization of Ascaris suum.
  • Purification of SOD using differential centrifugation, ammonium sulfate precipitation, ion-exchange chromatography, and G-75 Sephadex chromatography.

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  • Electrophoretic analysis of SOD forms.
  • Assay of SOD activity in various tissues.

    • Main Results:

    • Three distinct SOD forms were identified in Ascaris suum.
    • SOD forms exhibited different cellular localizations (cytoplasmic and mitochondrial) and sensitivities to cyanide and hydrogen peroxide.
    • Electrophoretic patterns clearly differentiated the SOD forms.
    • Highest SOD activities were observed in the female reproductive organs.

    Conclusions:

    • Ascaris suum possesses multiple SOD isoenzymes with distinct properties and locations.
    • The high SOD activity in reproductive tissues suggests a significant role in protecting these vital organs.
    • These findings underscore the critical role of superoxide dismutase in the metabolic processes of Ascaris suum.