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Potential applications of nanopore sequencing for forensic analysis
C L Hall1, R R Zascavage2, F J Sedlazeck3
1Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Centre, Fort Worth, Texas, United States of America.
Forensic Science Review
|February 3, 2020
Summary
Oxford Nanopore Technologies (ONT) offers a portable nanopore sequencing platform ideal for forensic analysis. This technology enables direct analysis of forensic samples, supporting various applications for laboratories of all sizes.
Area of Science:
- Genomics
- Forensic Science
- Biotechnology
Background:
- Rapid advancements in DNA sequencing technologies impact health and science research.
- Single-molecule sequencing platforms are emerging as valuable tools in diverse fields.
- Oxford Nanopore Technologies (ONT) developed a portable nanopore-based sequencing instrument.
Purpose of the Study:
- To outline recent developments and applications of nanopore sequencing technologies.
- To explore the potential of ONT sequencing in forensic analysis.
- To discuss its suitability for small to midsize forensic laboratories.
Main Methods:
- Review of current nanopore sequencing applications.
- Analysis of potential forensic applications including DNA and microbial identification.
- Discussion of library preparation and data analysis tools.
Main Results:
- ONT sequencing has been used for on-site virus identification and full genome sequencing.
- The platform shows promise for direct analysis of forensic samples (blood, semen, buccal swabs).
- Potential applications include mitochondrial DNA analysis, SNP/STR typing, and microbial identification.
Conclusions:
- The portable, low-cost ONT platform is well-suited for small forensic laboratories.
- Nanopore sequencing offers versatile applications in standard DNA sequencing and forensic analysis.
- Streamlined workflows and data analysis tools enhance the integration of nanopore technology in labs and field scenarios.
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