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Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus
Mayumi Tamura1, Toru Tanaka2, Norihiko Fujii3
1Faculty of Pharma-Science, Teikyo University.
Biological & Pharmaceutical Bulletin
|February 4, 2020
Summary
Galectin-2 (Gal-2) interacts with MUC5AC in mouse gastric mucus. This interaction, dependent on β-galactosides, strengthens the protective mucosal barrier.
Area of Science:
- Biochemistry
- Molecular Biology
- Gastroenterology
Background:
- Galectins are a protein family binding β-galactosides.
- Galectin-2 (Gal-2) is primarily found in the gastrointestinal tract.
- Gastric mucus provides a protective barrier for the stomach lining.
Purpose of the Study:
- To determine if Gal-2 is secreted by epithelial cells into gastric mucus.
- To identify ligands for Gal-2 within the gastric mucus.
- To investigate the functional significance of Gal-2-ligand interactions in gastric mucus.
Main Methods:
- Detection of Gal-2 in mouse gastric mucous fractions.
- Lactose elution to confirm β-galactoside binding.
- Affinity chromatography with recombinant mouse Gal-2 (mGal-2).
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for ligand identification.
- Western blotting to detect MUC5AC and mGal-2 binding.
Main Results:
- Gal-2 was detected in mouse gastric mucus and eluted by lactose.
- MUC5AC was identified as a potential ligand for mGal-2.
- mGal-2 binding to MUC5AC in gastric mucus was carbohydrate-dependent.
- These findings suggest a Gal-2 and MUC5AC interaction in gastric mucus.
Conclusions:
- Galectin-2 interacts with MUC5AC in mouse gastric mucus.
- This interaction is dependent on β-galactoside binding.
- The Gal-2/MUC5AC interaction enhances the gastric mucosal barrier structure.
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