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Updated: Dec 29, 2025

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Multiplex protein-specific microscopy with ultraviolet surface excitation.

Jiaming Guo^1,2, Camille Artur^1,2, Tasha Womack³

¹University of Houston, Department of Electrical and Computer Engineering, Houston, TX 77004, USA.

Biomedical Optics Express

|February 4, 2020

Summary

This summary is machine-generated.

Microscopy with ultraviolet surface excitation (MUSE) now enables protein-specific imaging using quantum dots. This innovation expands MUSE applications for complex diagnoses without tissue fixation or sectioning.

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Area of Science:

Biomedical Imaging
Nanotechnology
Molecular Pathology

Background:

Immunofluorescence (IF) staining is vital for protein expression analysis in tissues, aiding disease understanding and diagnosis.
Microscopy with ultraviolet surface excitation (MUSE) offers rapid imaging without fixation or sectioning but lacks integration into protein-specific IF workflows.
Current MUSE limitations hinder its use in complex diagnostics requiring specific protein markers.

Purpose of the Study:

To integrate MUSE with multiplex immunohistochemistry for protein-specific imaging.
To demonstrate the utility of quantum dot nanoparticles as labels for MUSE.
To expand MUSE applicability to both intact and processed tissue samples.

Main Methods:

Utilized quantum dot nanoparticles as fluorescent labels for protein detection.
Applied MUSE technology with ultraviolet excitation (≈280 nm) to tissue samples.
Evaluated imaging on both paraffin-embedded and intact tissue specimens.

Main Results:

Successfully demonstrated protein-specific MUSE imaging using quantum dots.
Achieved high-quality imaging comparable to traditional methods, without fixation or sectioning.
Expanded MUSE capabilities for multiplex immunohistochemistry applications.

Conclusions:

Quantum dot labels significantly broaden MUSE applicability for protein-specific analysis.
This advancement enables complex molecular profiling and diagnostics using MUSE.
Integration with 3D ultraviolet fluorescence microscopy promises future 3D IF imaging capabilities.