Related Experiment Video
Updated: Dec 29, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Stable multi-megahertz circular-ranging optical coherence tomography at 1.3 µm.
Norman Lippok1,2, Brett E Bouma1,2,3, Benjamin J Vakoc1,2,3
1Harvard Medical School, Boston, MA 02115, USA.
This study introduces a new circular-ranging optical coherence tomography (CR-OCT) system that overcomes previous stability and wavelength limitations. The enhanced CR-OCT design enables deeper imaging in tissues and broader applications in medicine and beyond.
Area of Science:
- Biomedical Engineering
- Optical Physics
- Medical Imaging
Background:
- Fourier-domain optical coherence tomography (OCT) systems face depth and speed limitations due to finite electronic bandwidth.
- Existing circular-ranging OCT (CR-OCT) methods offer improved range but suffer from instability and operate at suboptimal wavelengths (1.55 µm).
Purpose of the Study:
- To develop a novel CR-OCT architecture that enhances stability, simplifies design, and shifts the operating wavelength for improved biological tissue penetration.
- To overcome the limitations of previous CR-OCT systems, enabling wider applicability.
Main Methods:
- Replaced temperature-sensitive optical modules and eliminated long fiber spools in favor of a 10-meter chirped fiber Bragg grating (CFBG).
- Implemented an active quadrature demodulation circuit using a lithium niobate phase modulator.
- Shifted the operating wavelength to 1.29 µm using CFBG-based dispersive fibers for enhanced tissue imaging.
Main Results:
- Achieved a stable CR-OCT system with a simplified design.
- Demonstrated operation at 1.29 µm, improving imaging penetration.
- Maintained a broad 100 nm optical bandwidth, a 4 cm imaging range, and a high 7.6 MHz A-line rate.
Conclusions:
- The novel CR-OCT architecture significantly enhances stability and simplifies system design compared to previous methods.
- Operating at 1.29 µm allows for deeper imaging in biological tissues.
- This improved CR-OCT system is poised for broader exploration in medical and non-medical fields.
More Related Videos
12:22Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
Published on: August 4, 2018
08:50Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Published on: February 9, 2019
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Two-Dimensional Microscopy in Microbiology
Three-Dimensional Microscopy in Microbiology
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Total Internal Reflection Fluorescence Microscopy