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Antibiogram and genetic relatedness of clinical isolates of Enterococcus spp. in Mangalore, India

Prathvi P Nayak1, Dhanashree Biranthabail2, Shalini Shenoy3

  • 1Manipal Academy of Higher Education, Manipal, India. prathvip.nayak@gmail.com.

Journal of Infection in Developing Countries
|February 4, 2020
PubMed
Abstract

Insights

Drug-resistant Enterococcus species pose a significant clinical challenge. This study in India reveals baseline resistance patterns and genetic relatedness, highlighting their role as reservoirs for antibiotic-resistant genes.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Drug-resistant Enterococcus species present a persistent clinical problem.
  • These bacteria can act as reservoirs for antibiotic-resistant genes.
  • Understanding local resistance patterns is crucial for effective treatment.

Purpose of the Study:

  • To determine the antibiogram of Enterococcus species isolated from clinical samples.
  • To investigate the genetic relatedness of these isolates.
  • To establish baseline resistance data in Mangalore, India.

Main Methods:

  • Antimicrobial susceptibility testing using the Kirby Bauer disk diffusion method.
  • Molecular typing of isolates via Random Amplification of Polymorphic DNA (RAPD).
  • Analysis of 150 non-repetitive Enterococcus isolates from urine, pus, and blood samples.

Main Results:

  • Enterococcus faecium from urine showed higher resistance than from pus (p < 0.001).
  • Blood isolates of E. faecium and E. faecalis exhibited resistance to common antibiotics like ampicillin, penicillin, and ciprofloxacin.
  • All isolates were sensitive to vancomycin; 44 isolates showed high-level aminoglycoside resistance (HLAR).
  • RAPD analysis revealed similarities and differences in banding patterns, indicating genetic diversity.

Conclusions:

  • The study identified baseline resistance among Enterococcus species in the studied area.
  • This resistance poses a challenge for clinicians.
  • Enterococcus isolates serve as a reservoir for the transmission of antibiotic-resistant genes.
Keywords:
EnterococcusHLARRAPDmolecular typingmultidrug resistance

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