Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

784
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
784

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimisation of the crystallisation process through staggered cooling in a nonvibrating granular system.

Scientific reports·2025
Same author

Impact of universal use of the McGrath videolaryngoscope as the first option for all intubations in the operating room: The multicentre prospective before-after VIDEOLAR-SURGERY study protocol.

Revista espanola de anestesiologia y reanimacion..·2024
Same author

Double vitrification and warming does not compromise the chance of live birth after single unbiopsied blastocyst transfer.

Human reproduction open·2023
Same author

Two-step crystallisation in a 2D active magnetic granular system confined by a parabolic potential.

Scientific reports·2023
Same author

Higher miscarriage rate in subfertile women with endometriosis receiving unbiopsied frozen-warmed single blastocyst transfers.

Frontiers in cell and developmental biology·2023
Same author

Neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio in depressed patients with suicidal behavior: A systematic review.

European psychiatry : the journal of the Association of European Psychiatrists·2023

Related Experiment Video

Updated: Dec 29, 2025

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
08:17

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

11.1K

Optimization of the Preanalytical Phase by Estimating Serum Indices Using an Automated Classifier.

O Herráez, A Velasco, A Escobar

    Clinical Laboratory
    |February 5, 2020
    PubMed
    Summary

    This study validates the RSD classifier for estimating serum indices, reducing unnecessary lab tests and saving over €15,000 annually. It also identified new interferences affecting iron, transferrin, fibrinogen, and alkaline phosphatase.

    More Related Videos

    Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
    09:32

    Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

    Published on: September 10, 2017

    11.4K
    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
    09:00

    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

    Published on: October 31, 2012

    16.2K

    Related Experiment Videos

    Last Updated: Dec 29, 2025

    A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
    08:17

    A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

    Published on: February 8, 2016

    11.1K
    Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
    09:32

    Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

    Published on: September 10, 2017

    11.4K
    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
    09:00

    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

    Published on: October 31, 2012

    16.2K

    Area of Science:

    • Clinical Chemistry
    • Laboratory Medicine
    • Data Science in Healthcare

    Background:

    • Preanalytical errors significantly impact laboratory testing accuracy.
    • Hemolysis, lipemia, and icterus are common preanalytical interferences.
    • Accurate identification of these interferences is crucial for reliable results.

    Purpose of the Study:

    • To evaluate the RSD classifier for serum interference estimation.
    • To identify additional biochemical parameters affected by preanalytical interferences.
    • To determine the economic impact of implementing this methodology.

    Main Methods:

    • Collected serum indices from 65,529 requests using the RSD system and Cobas platforms.
    • Employed data mining to find associations between serum indices and laboratory tests.
    • Evaluated interference impact on 91 biochemistry, immunoassay, and coagulation tests.

    Main Results:

    • The RSD model demonstrated 94.4% accuracy in compatibility with analytical methods.
    • Implementation of RSD-based serum indices estimation could save €10,561 annually by avoiding unnecessary tests.
    • Identified iron, transferrin, fibrinogen, and alkaline phosphatase as tests affected by hemolysis.

    Conclusions:

    • The RSD classifier is an effective method for estimating serum indices and bilirubin values.
    • Implementing this methodology can save over €15,000 annually without increasing turnaround times.
    • Iron, alkaline phosphatase, transferrin, and fibrinogen should be considered in interference evaluation protocols.