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CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Dec 29, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

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Published on: November 1, 2024

RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering.

Aaron A Smargon¹, Yilan J Shi^1,2, Gene W Yeo^3,4

¹Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.

Nature Cell Biology

|February 5, 2020

Summary

RNA-targeting CRISPR-Cas (RCas) technology is revolutionizing biology by enabling precise RNA manipulation. This perspective explores RCas advancements, applications in RNA biology, and future research directions.

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Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

CRISPR-Cas technology has significantly advanced biological research.
Exploring functional diversity reveals new CRISPR-Cas systems and applications.
RNA manipulation is a rapidly growing area within CRISPR-based engineering.

Purpose of the Study:

To provide a comprehensive perspective on the progress of RNA-targeting CRISPR-Cas (RCas) systems.
To highlight the translation of scientific discoveries into practical applications for RNA biology.
To identify current challenges and future opportunities in the RCas field.

Main Methods:

Review and synthesis of current literature on RNA-targeting CRISPR-Cas systems.
Analysis of emerging applications in RNA biology and biotechnology.
Discussion of future research directions and potential technological advancements.

Main Results:

Significant progress has been made in developing and applying RNA-targeting CRISPR-Cas technologies.
RCas systems offer powerful tools for manipulating RNA, impacting various biological processes.
The field is rapidly evolving, with ongoing discovery of new systems and applications.

Conclusions:

RNA-targeting CRISPR-Cas technology represents a powerful and versatile platform for RNA biology research and engineering.
Continued exploration of RCas systems promises further breakthroughs in understanding and manipulating RNA.
Addressing current obstacles and exploring alternative technologies will drive the future of RCas applications.