Related Experiment Video
Updated: Dec 29, 2025

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
MS-Derived Isotopic Fine Structure Reveals Forazoline A as a Thioketone-Containing Marine-Derived Natural Product
Fan Zhang1, Thomas P Wyche2, Yanlong Zhu3,4
1Pharmaceutical Sciences Division , University of Wisconsin-Madison , Madison , Wisconsin 53705 , United States.
Abstract:
Forazoline A is a structurally complex PKS-NRPS hybrid produced by marine-derived Actinomadura sp. During the course of studies highlighting the application of IFS analysis as a powerful tool for natural products analysis, we were alerted to an earlier misinterpretation with respect to forazoline A structure elucidation. In particular, IFS reveals that forazoline A contains a thioketone moiety rarely seen in secondary metabolites and, thus, constitutes an even more intriguing structure than originally thought.
Related Concept Videos
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Mass Spectrum: Interpretation
Mass Spectrometry: Long-Chain Alkane Fragmentation
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
Mass Spectrometry: Branched Alkane Fragmentation
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

