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Updated: Dec 29, 2025

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

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Materials and Microenvironments for Engineering the Intestinal Epithelium.

Jessica Snyder¹, Chia-Ming Wang¹, An Qi Zhang²

¹Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA.

Annals of Biomedical Engineering

|February 6, 2020

Summary

This summary is machine-generated.

Developing advanced in vitro models of the gastrointestinal epithelium is key for studying intestinal homeostasis. These models aim to replicate complex cell interactions and functions for better disease research.

Keywords:

Biomaterial Enteric Epithelium Immune Microfluidics Stroma

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Area of Science:

Gastroenterology and Regenerative Medicine
Biomaterials Science
Cell Biology

Background:

The gastrointestinal tract's barrier function depends on intestinal epithelial cells, which maintain homeostasis by mediating signals between stromal cells and luminal factors.
Developing accurate in vitro models of the gastrointestinal epithelium is vital for understanding intestinal cell interactions in health and disease.
Ideal models should incorporate microbiota, immune cells, polarized architecture, extracellular matrix, and mechanical stimuli.

Purpose of the Study:

To review historical and current biomaterials and substrates for creating static and fluidic in vitro intestinal models.
To discuss the use of various cell types, including colon cancer cell lines, organoid-derived epithelial cells, and stromal support cells.
To highlight the importance of increasing cellular complexity in these models and outline future directions.

Main Methods:

Review of literature on biomaterials and substrates for intestinal epithelial models.
Analysis of cell types used in in vitro gastrointestinal models (cancer cell lines, primary cells, stromal cells).
Discussion of static and fluidic model systems and their components.

Main Results:

Biomaterials and substrates have evolved for creating increasingly representative intestinal epithelial models.
A range of cell sources, from cancer cell lines to primary organoid cells and diverse stromal cells, are employed.
Static and fluidic systems offer different advantages for recapitulating intestinal functions.

Conclusions:

Advancements in biomaterials and cell sourcing are enabling more sophisticated in vitro models of the intestinal epithelium.
Incorporating greater cellular complexity and mimicking physiological conditions are crucial for future model development.
These improved models hold significant promise for advancing research in intestinal homeostasis and disease.