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Updated: Dec 29, 2025
Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
Published on: February 13, 2019
Two novel alkaloids from Corydalis curviflora Maxim. and their insecticidal activity
JianKai Xia1, Yao Liu1, Yu Zhou2
1Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, China.
Background:
Botanical pesticide plays an essential role in the control of agricultural pests. Corydalis curviflora Maxim. is used as a cholagogue and larvicide in the rural areas of Northwest China. In this study, our objective was to identify the insect active ingredients of C. curviflora extract.
Results:
Bioassay-guided isolation of the high active fraction led to the identification of two novel N-demethyl hexahydrobenzophenanthridine-type alkaloids, Curviflorain A (1) and Curviflorain B (2), together with nine known alkaloids, ambiguanine A (3), ambiguanine B (4), ambiguanine C (5), 6-acetylambinine (6), 1,1-dimethyl-6-methoxy-7-hydroxyl-1,2,3,4-tetrahydroisoquinoline (7), hendersine B (8), coryximine (9), isochotensine (10) and corysolidine (11). Compounds 1, 2, and 6 showed promising activity to the larvae of Culex pipiens pallens Coq. and Aedes albopictus Skuse. These compounds were also tested against the insect pests, Mythimna separata walker. and Schizaphis graminum Rondani.
Conclusion:
These findings provide a better understanding of the insecticidal activity of C. curviflora extract and the active compounds. This has the potential to lead to a more effective botanical insecticide.
