A Multi-Channel System for Temperature Sensing of Neural Stem Cells in Adherent Culture

Neural stem cells (NSCs) can gradually proliferate or differentiate during adherent culture. It is found that stem cells have different temperature characteristics in different physiological states. In order to detect the temperature of NSCs during adherent culture, in this study, we have designed a temperature monitoring system, in which a thin-film platinum resistor was used as the sensor. The NSCs were seeded on the sensor, and the data acquisition device was connected to the host computer via Bluetooth. Results indicate that there are about 5000 cells attached on the surface of each sensor, and the cell viability is maintained at about 90% after 24 h culture. An electrostatic force microscope (EFM) result proves that there is no electric field on the sensor surface to influence the activity of NSCs. This system can work continuously for more than 24 h with 0.05 °C detection sensitivity. Furthermore, the significant temperature change of NSCs is observed when stimulated by different concentrations of thyroid hormone, which demonstrates that the temperature change related to cell activity. Therefore, by detecting the temperature of the cell population, the fabricated system can provide reference information for studying the metabolic state of NSCs, as well as physiological responses of cells under various conditions in biomedical applications.