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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Identification of a Unique Azaphilone Produced by Chaetomium globosum Isolated from Polygonatum sibiricum
Chenggang Song1, Gang Ding2, Gang Wu1
1College of Plant Science, Jilin University, Changchun, 130062, P. R. China.
Abstract:
A new azaphilone, chaephilone E, eight azaphilone derivatives, and three chaetoglobosins were isolated from endophytic fungi Chaetomium globosum. The structures of the compounds were elucidated by 1D and 2D NMR as well as HR-ESI-MS data, and the absolute configuration of chaephilone E was established on the basis of electronic circular dichroism and NOESY spectrum. The activity of chaephilone E was evaluated via the cytotoxic assay (human hepatoma cell lines HepG-2) and brine shrimp (Artemia salina) bioassay.
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