Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.5K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.5K
Homologous Recombination02:31

Homologous Recombination

62.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.1K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.5K
CRISPR01:59

CRISPR

57.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FEASIBILITY AND INTERIM SAFETY AND EFFICACY ANALYSIS OF A FAST AUTOMATED PRODUCTION OF PROPHYLACTIC CMV-SPECIFIC CTLs AFTER ALLOGENEIC HSCT.

Transplantation and cellular therapy·2026
Same author

Stalled Progress in HIV Prevention for Trans Women in San Francisco: A Call for Upstream Solutions and Renewed Support for Public Health and Human Rights.

AIDS and behavior·2026
Same author

Multi-level Risks Associated with Partner and Client Violence Among Trans Women Who Do Sex Work in San Francisco, 2023-2024.

AIDS and behavior·2026
Same author

Oropouche virus RNA persistence and antibody kinetics: a multi-specimen, genomic and serological analysis.

Journal of travel medicine·2026
Same author

Understanding the role of overdose witnesses: responses and characteristics of people who inject drugs in Oakland and San Francisco.

Harm reduction journal·2026
Same author

Shifting Patterns of Injection Drug Use in San Francisco, United States: implications for Getting to Zero HIV Infections.

Substance use & misuse·2026

Related Experiment Video

Updated: Dec 29, 2025

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K

New Bicistronic TALENs Greatly Improve Genome Editing.

José María Martín-Fernández1, Aarne Fleischer1,2, Sara Vallejo-Diez2

  • 1Karuna Good Cells Technologies SL, Vitoria-Gasteiz, Álava, Spain.

Current Protocols in Stem Cell Biology
|February 6, 2020
PubMed
Summary

Researchers developed a more efficient transcription activator-like effector nuclease (TALEN) system for gene editing. This novel TALEN approach streamlines cell selection, accelerating stem cell and regenerative medicine research.

Keywords:
FACS selectionMACS selectionTALENsbicistronic transcription activator-like effector nucleasegenome editing

More Related Videos

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.1K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.3K

Related Experiment Videos

Last Updated: Dec 29, 2025

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.1K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.3K

Area of Science:

  • Molecular Biology
  • Gene Editing Technologies

Background:

  • Genome editing is crucial in stem cell and regenerative medicine.
  • Current methods using transcription activator-like effector nucleases (TALENs) require improvement for efficiency.

Purpose of the Study:

  • To introduce a novel, highly efficient TALEN system for gene editing.
  • To improve the selection process for successfully edited cells.

Main Methods:

  • Generation of bicistronic TALEN genes linked to reporter molecules (TALEN-F, TALEN-M).
  • Utilizing fluorescence-assisted cell sorting (FACS) or magnetic-activated cell sorting (MACS) for reporter-based selection.
  • Application in primary T cell editing.

Main Results:

  • The bicistronic TALEN system enables rapid enrichment of cells with active TALEN pairs.
  • Eliminates the need for extensive culture selection and clone characterization.
  • Demonstrates successful editing of primary T cells.

Conclusions:

  • The new TALEN system significantly enhances gene editing efficiency and cell selection.
  • This advancement accelerates research in stem cell and regenerative medicine.
  • Facilitates faster generation and characterization of edited cell populations.