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Matrix Metalloproteinase 13 Activity is Required for Normal and Hypoxia-Induced Precocious Hatching in Zebrafish
Christopher D Small1, Megan El-Khoury1, Ghislain Deslongchamps2
1Biology Department, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.
Journal of Developmental Biology
|February 7, 2020
Summary
Matrix metalloproteinase 13a (Mmp13a) is crucial for zebrafish hatching. Its activity is necessary for both normal and hypoxia-induced precocious hatching, likely by activating the hatching enzyme.
Area of Science:
- Developmental Biology
- Molecular Biology
- Zebrafish Embryology
Background:
- Hypoxia triggers precocious hatching in zebrafish.
- Molecular mechanisms of hatching enzyme activation under stress are unclear.
Purpose of the Study:
- Investigate the role of matrix metalloproteinase 13a (Mmp13a) in zebrafish hatching.
- Elucidate the molecular mechanisms of hypoxia-induced precocious hatching.
Main Methods:
- Immunohistochemistry to localize Mmp13a.
- Pharmacological inhibition of Mmp13a.
- In vivo zymography to assess enzyme activity.
Main Results:
- Mmp13a is present in the hatching gland during hatching competence.
- Mmp13a activity is essential for normal and precocious hatching.
- Mmp13a is necessary, but not sufficient, for zebrafish hatching.
Conclusions:
- Mmp13a likely activates the hatching enzyme zymogen.
- Mmp13a plays a key role in extra-embryonic processes during development.
- Highlights the diverse functions of matrix metalloproteinases (MMPs) in development.
