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From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

Rosita A Condorelli1, Aldo E Calogero1, Giorgio I Russo2

  • 1Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy.

Journal of Clinical Medicine
|February 8, 2020
PubMed
Summary
This summary is machine-generated.

Evaluating bio-functional sperm parameters alongside conventional analysis can help explain male infertility. Key parameters like mitochondrial membrane potential correlate with sperm quality, aiding diagnosis in unexplained cases.

Keywords:
bio-functional sperm parametersmale infertilitysperm mitochondrial functionsperm parameters

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Area of Science:

  • Reproductive Biology
  • Andrology
  • Spermatozoa Functionality

Background:

  • Conventional semen analysis has limitations in diagnosing male infertility.
  • Bio-functional sperm parameters offer deeper insights into sperm quality and fertilizing potential.

Purpose of the Study:

  • To evaluate the correlation between conventional and bio-functional sperm parameters in infertile patients.
  • To identify thresholds of bio-functional parameters associated with conventional sperm abnormalities.

Main Methods:

  • Analysis of 577 infertile patients with infertility lasting at least 12 months.
  • Evaluation of mitochondrial membrane potential (MMP), sperm viability, and chromatin abnormality using flow cytometry.
  • Correlation analysis between conventional and bio-functional sperm parameters based on percentile distribution.

Main Results:

  • High MMP (≥46.25%) correlated positively with sperm concentration, count, progressive motility, and normal form.
  • Abnormal chromatin compactness (≥21.10%) showed reduced risk with increased total sperm count and motility.
  • Specific cut-off values were identified for MMP, viability, and chromatin abnormality, linking them to conventional parameter deviations.

Conclusions:

  • Bio-functional sperm parameters are significantly correlated with conventional semen analysis results.
  • Flow cytometry evaluation of bio-functional parameters can aid in diagnosing idiopathic male infertility.
  • These findings suggest improved diagnostic capabilities for male infertility by integrating bio-functional assessments.