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Molecular cloning of bovine beta-lactoglobulin cDNA
V N Ivanov1, E S Judinkova, S I Gorodetsky
1N.I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.
Biological Chemistry Hoppe-Seyler
|June 1, 1988
Abstract:
A cDNA library from bovine mammary gland mRNA was constructed in pBR322 and screened by hybrid-selected translation and immunoscreening. Several beta-lactoglobulin clones were identified and sequenced. All clones contained cDNA fragments corresponding to the 3' region of beta-lactoglobulin mRNA. The 3' non-translated region of beta-lactoglobulin mRNA consists of 187 nucleotides; the polyadenylation signal AATAAA occurs 17 nucleotides before the poly(A) tail. The amino-acid sequence predicted from the 3' coding region corresponds completely to the previously determined amino-acid sequence of beta-lactoglobulin.