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Related Concept Videos

Tight Junctions01:29

Tight Junctions

6.7K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
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Clinical Applications of Epidermal Stem Cells01:19

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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Related Experiment Video

Updated: Dec 29, 2025

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
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Claudin-1 decrease impacts epidermal barrier function in atopic dermatitis lesions dose-dependently.

Sophia Bergmann1, Barbara von Buenau1, Sabine Vidal-Y-Sy1

  • 1Department of Dermatology and Venerology, University Hospital Hamburg-Eppendorf, Hamburg, Germany.

Scientific Reports
|February 8, 2020
PubMed
Summary
This summary is machine-generated.

Reduced claudin-1 levels in atopic dermatitis (AD) impair skin barrier function and promote inflammation. Increasing claudin-1 may offer a therapeutic target for AD by restoring barrier integrity and reducing inflammation.

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Area of Science:

  • Dermatology
  • Cell Biology
  • Immunology

Background:

  • Claudin-1 is a key protein in epidermal tight junctions (TJs), forming a crucial skin barrier.
  • Claudin-1 downregulation is implicated in atopic dermatitis (AD), but its precise role and levels in healthy skin are variable.
  • Understanding claudin-1's impact across different levels is vital for AD pathogenesis and treatment.

Purpose of the Study:

  • To investigate the correlation between claudin-1 levels and skin barrier function in healthy individuals and AD patients.
  • To determine the functional consequences of varying claudin-1 expression on epidermal barrier integrity and inflammatory responses.
  • To explore the potential of claudin-1 as a therapeutic target for AD.

Main Methods:

  • Quantified claudin-1 levels in AD patients and controls, correlating them with TJ and skin barrier function.
  • Utilized reconstructed human epidermis models with varying claudin-1 expression.
  • Assessed epidermal barrier function using molecular tracers and evaluated inflammatory responses to bacteria.

Main Results:

  • Healthy skin exhibited a wide range of claudin-1 levels with stable barrier function.
  • Lesional AD skin showed significantly reduced claudin-1 levels and impaired TJ barrier function.
  • Lower claudin-1 levels correlated with compromised barrier function and induced keratinocyte IL-1β expression, fostering inflammation.

Conclusions:

  • Claudin-1 levels below a critical threshold impair epidermal barrier function and trigger inflammation.
  • Restoring claudin-1 levels may enhance skin barrier function and reduce inflammation in AD.
  • Claudin-1 represents a promising therapeutic target for managing atopic dermatitis.