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Updated: Dec 29, 2025
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
A β-allyl carbamate fluorescent probe for vicinal dithiol proteins
Lanning Zhao1, Feifei Bai1, Fan Chen1
1State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, China. fangjg@lzu.edu.cn.
Abstract:
The first β-allyl carbamate fluorescent probe, AC-green, was reported for specifically imaging vicinal dithiol proteins (VDPs) in living systems. The probe displays low toxicity and features high sensitivity and fast response towards VDPs. A drastic loss of VDPs in a Parkinson's model was disclosed by AC-green, linking the fluctuation of VDP levels to Parkinson's disease for the first time. The structural novelty and favorable properties of AC-green will advance the development of novel VDP probes.
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