β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
Camila P Favaro1,2, Ilton J Baraldi3, Fernanda P Casciatori2
1Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP 13560-970, Brazil.
Biomolecules
|February 9, 2020
Summary
Enzymes produced from coffee waste using solid-state fermentation (SSF) effectively hydrolyze coffee polysaccharides. This sustainable method enhances soluble coffee processing and promotes a circular economy in the coffee industry.
Area of Science:
- Biotechnology
- Food Science
- Industrial Microbiology
Background:
- Soluble coffee processing can be improved through enzymatic hydrolysis of coffee polysaccharides.
- Biochemical routes using enzymes offer an eco-friendly and sustainable alternative to traditional methods.
- Utilizing coffee industry waste as a substrate for enzyme production aligns with circular economy principles.
Purpose of the Study:
- To produce hydrolytic enzymes via solid-state fermentation (SSF) using coffee industry waste.
- To apply these enzymes for the hydrolysis of polysaccharides in soluble coffee processing.
- To optimize enzyme production and assess its efficacy in enhancing coffee quality and sustainability.
Main Methods:
- Filamentous fungi were cultivated on coffee waste and wheat bran mixtures for enzyme production via SSF.
- Enzyme production was optimized and compared between flasks and a packed-bed bioreactor.
- The produced enzymatic extract was characterized for stability and applied to hydrolyze coffee polysaccharides, with and without a commercial cellulase cocktail.
Main Results:
- A packed-bed bioreactor yielded higher enzyme production compared to flasks due to improved aeration.
- The optimal substrate for β-mannanase production by *Aspergillus niger* F12 was a 1:1 (w/w) mixture of coffee waste and wheat bran.
- The enzymatic extract demonstrated stability at 50 °C for 24 hours and significantly hydrolyzed coffee carbohydrates, with a 56% increase in yield when combined with a commercial cellulase cocktail.
Conclusions:
- Solid-state fermentation of coffee waste is an effective method for producing valuable hydrolytic enzymes like β-mannanase.
- The application of these enzymes shows significant potential for improving soluble coffee processing, reducing energy requirements, and minimizing environmental impact.
- This approach supports the development of a circular economy within the coffee industry by valorizing waste streams.
