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Updated: Dec 29, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Evaluation of filter paper as a means to transport inactivated bacteria for identification using the MALDI-TOF MS
M S Carneiro1, A Fracasso2, O V A Lovison1
1Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro De Pesquisa Experimental (CPE), Hospital de Clínicas de Porto Alegre (HCPA), R. Ramiro Barcelos, 2350, CEP 90035-903 Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, Porto Alegre, Brazil.
Abstract:
A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternative to transport bacteria for MALDI-TOF MS identification.

