Fifth generation troponin T assay is subject to antibody interference
Jeffrey Hedley1, Venu Menon1, Leslie Cho1
1Heart and Vascular Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Background:
The fifth generation (high-sensitivity) troponin T assay offers increased precision and analytical sensitivity to the predecessor method. The assay has proven utility in risk stratification and patient management. Upon clinical suspicion and discordant 4th generation troponin T and troponin I results, we investigated a sample for suspected interfering substances to the 5th generation troponin T assay.
Methods:
The analysis included a serial dilution, treatment with polyethylene glycol, commercial antibody blocking reagents, and size exclusion chromatography.
Results:
The sample diluted linearly (R2 = 0.9957); however, experienced a dramatic reduction in concentration after both the polyethylene glycol and blocking agent treatment. Finally, size exclusion chromatography demonstrated assay reactivity around 970 kDa range.
Conclusions:
These experiments elucidate a heterophilic antibody interference to the assay, and demonstrate potential measures to discern the interference.
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