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Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Related Experiment Video

Updated: Dec 29, 2025

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Automated access to well-defined ionic oligosaccharides.

Yuntao Zhu¹, Theodore Tyrikos-Ergas², Kevin Schiefelbein¹

¹Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany. martina.delbianco@mpikg.mpg.de.

Organic & Biomolecular Chemistry

|February 11, 2020

Summary

This summary is machine-generated.

Automated synthesis of novel ionic oligosaccharides using unique monosaccharides enables structural characterization. This research clarifies how charge patterns influence glycan conformation, advancing polysaccharide science.

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Last Updated: Dec 29, 2025

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Area of Science:

Carbohydrate Chemistry
Glycobiology
Polymer Science

Background:

Ionic polysaccharides play crucial roles in biological processes.
Structural characterization of these molecules is hindered by difficult purification and synthesis.
Understanding their structure is key to elucidating their biological functions.

Purpose of the Study:

To develop an automated synthesis method for ionic oligosaccharides.
To characterize the structure of synthesized ionic oligosaccharides.
To investigate the impact of charge patterns on glycan conformation.

Main Methods:

Automated synthesis utilizing four unique, non-natural monosaccharides.
Advanced structural analysis techniques to determine oligosaccharide architecture.
Conformational analysis to correlate charge distribution with molecular shape.

Main Results:

Successful automated synthesis of a diverse library of ionic oligosaccharides.
Detailed structural elucidation of the synthesized compounds.
Demonstrated correlation between ionic charge patterns and distinct glycan conformations.

Conclusions:

Automated synthesis offers a viable route to structurally complex ionic polysaccharides.
Charge distribution is a critical determinant of ionic glycan conformation.
This work provides a foundation for designing functional ionic glycans.