An in vitro Raman study on compositional correlations of lipids and protein with animal tissue hydration

Raman spectroscopy is a powerful non-invasive tool for detection and classification of chemical composition of materials including biological tissues. In this work, we report an in vitro Raman study on animal skin samples with a focus on high-frequency vibrations such as symmetric CH₃ stretching mode at 2934 cm^-1, and the symmetric CH₂ vibration mode at 2854 cm^-1, OH stretching modes near 3412 cm^-1, and bounded OH mode near 3284 cm^-1. Raman data was acquired with a customized InGaAs based Raman spectrometer that consolidates the NIR (866 nm) light and the InGaAs detector and is particularly suitable for probing high-frequency vibrations. The Raman spectra of fat, tendon, and muscle tissues are also analyzed to determine the spectroscopic identities of CH and OH groups in skin. Our results suggest that the protein is beneficial for the maintenance of skin hydration, as it has higher water capacity and greater capability to retain water than lipids. This conclusion is consistent with the additional discovery that water exists in fat mainly as unbound type, while part of water exists as bound type in muscle.