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Updated: Dec 28, 2025
06:09
Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
Published on: November 8, 2021
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Mitochondrial dynamics and metabolism in induced pluripotency
Javier Prieto1, Xavier Ponsoda2, Juan Carlos Izpisua Belmonte3
1Departamento Biología Celular, Biología Funcional y Antropología Física, Universitat de València, Calle Dr. Moliner 50, 46100 Burjassot, Spain; Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Experimental Gerontology
|February 12, 2020
Summary
Somatic cells can be reprogrammed to pluripotency using factors or chemicals, undergoing significant cellular changes. This review discusses how these transformations are controlled to achieve a pluripotent state for stem cell development.
Area of Science:
- Cell Biology
- Stem Cell Research
- Epigenetics
Background:
- Somatic cells can be induced to pluripotency via genetic factors or chemical compounds.
- Cellular reprogramming involves extensive alterations in metabolism, mitochondrial function, and signaling pathways.
Purpose of the Study:
- To review recent data on the control of cellular processes during somatic cell reprogramming.
- To elucidate the mechanisms underlying the phenotypic transformation into pluripotent stem cells.
Main Methods:
- Literature review of recent studies on cell reprogramming.
- Analysis of data from various laboratories investigating cellular changes during reprogramming.
Main Results:
- Reprogramming induces profound changes in somatic cell metabolism and mitochondrial dynamics.
- Regulation of these cellular processes is crucial for achieving and maintaining pluripotency.
- Reprogrammed cells exhibit indefinite self-renewal and differentiation potential.
Conclusions:
- Understanding the regulation of cellular processes is key to efficient somatic cell reprogramming.
- This review synthesizes current knowledge on the control mechanisms driving the transition to pluripotency.
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