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The evolution of SPHIRE-crYOLO particle picking and its application in automated cryo-EM processing workflows

Thorsten Wagner1, Stefan Raunser2

  • 1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany.

Communications Biology
|February 13, 2020
PubMed
Abstract

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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