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Updated: Dec 28, 2025

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
Published on: March 11, 2013
Emergence and Developmental Roles of the Cerebrospinal Fluid System
Ryann M Fame1, Maria K Lehtinen1
1Department of Pathology, Boston Children's Hospital, Boston, MA 02115, USA.
Abstract:
We summarize recent work illuminating how cerebrospinal fluid (CSF) regulates brain function. More than a protective fluid cushion and sink for waste, the CSF is an integral CNS component with dynamic and diverse roles emerging in parallel with the developing CNS. This review examines the current understanding about early CSF and its maturation and roles during CNS development and discusses open questions in the field. We focus on developmental changes in the ventricular system and CSF sources (including neural progenitors and choroid plexus). We also discuss concepts related to the development of fluid dynamics including flow, perivascular transport, drainage, and barriers.
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