Fabrication of refreshable aptasensor based on hydrophobic screen-printed carbon electrode interface
Jiayun Fu1, Haowei Dong2, Qingxue Zhao3
1School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 12 Zhangzhou Road, Zibo 255049, China.
The Science of the Total Environment
|February 14, 2020
Summary
A novel refreshable electrochemical aptasensor uses a hydrophobic electrode and magnetic nanocomposite to detect organophosphorus pesticides (OPs). This reusable sensor offers potential for sensitive, low-concentration small molecule analysis.
Area of Science:
- Electrochemistry
- Materials Science
- Biosensors
Background:
- Electrode passivation limits sensor reusability in electrochemical detection.
- Organophosphorus pesticides (OPs) pose significant environmental and health risks.
- Development of sensitive and reusable sensors is crucial for OPs monitoring.
Purpose of the Study:
- To develop a refreshable electrochemical aptasensor for organophosphorus pesticide (OPs) detection.
- To overcome electrode passivation issues for enhanced sensor reusability.
- To utilize a magnetic nanocomposite for efficient target capture and detection.
Main Methods:
- Fabrication of a hydrophobic electrode by modifying a screen-printed carbon electrode (SPCE) with polydimethylsiloxane (PDMS).
- Synthesis of a graphene oxide-ferroferric oxide (GO-Fe3O4) magnetic nanocomposite functionalized with aptamers (Apt).
- Electrochemical detection of OPs using magnetic separation and signal measurement on the hydrophobic electrode.
Main Results:
- The hydrophobic electrode demonstrated reusability for at least two detection cycles.
- Electrochemical signal changes correlated directly with OPs concentrations.
- The sensor successfully enabled qualitative evaluation of OPs in spiked vegetable samples.
- The magnetic capture system facilitated the detection of low-concentration OPs.
Conclusions:
- The developed refreshable aptasensor offers a promising platform for reusable electrochemical sensing.
- The combination of hydrophobic electrodes and magnetic nanocomposites enhances sensor performance and reusability.
- This approach holds significant potential for the sensitive detection and evaluation of small molecule targets like OPs.
