Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bipolar Electrochemiluminescence at Single Gold Microbeads Trapped by Micropipettes.

Chemical & biomedical imaging·2026

Same author

Dual-pathway sensing in Ti-MOFs for selective VOC discrimination by SERS.

Chemical communications (Cambridge, England)·2026

Same author

Evaluation of the Crushing Characteristics of Slate Coarse Aggregate Used for Asphalt Mixture.

Materials (Basel, Switzerland)·2026

Same author

Maackiain Reduces Neuroinflammation by Modulating Inflammatory Signals in LPS-Induced <i>In Vitro</i> and <i>In Vivo</i> Models.

Journal of microbiology and biotechnology·2026

Same author

Tse<sup>VF</sup>-Tsi<sup>VF</sup>, a novel bacteriolytic effector-immunity pair of <i>Vibrio fluvialis</i> VflT6SS2, provides a fitness advantage in microbial competition.

Current research in microbial sciences·2026

Same author

Individual Single-Crystalline Irregular In<sub>2</sub>O<sub>3</sub> Microcavity for Ultrasensitive Semiconductor-Based SERS Biosensor.

Advanced materials (Deerfield Beach, Fla.)·2026

Search research articles

Related Experiment Video

Updated: Dec 28, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Ratiometric Fluorescent Quantum Dot-Based Biosensor for Chlorothalonil Detection via an Inner-Filter Effect.

Enze Sheng¹, Yuxiao Lu¹, Yuting Tan²

¹Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.

Analytical Chemistry

|February 14, 2020

Summary

A novel sensor detects chlorothalonil (CHL) using gold nanoparticles and fluorescent quantum dots. This method effectively measures CHL in food and environmental samples with high sensitivity.

More Related Videos

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

Related Experiment Videos

Last Updated: Dec 28, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

Area of Science:

Nanotechnology
Analytical Chemistry
Biochemistry

Background:

Chlorothalonil (CHL) is a widely used fungicide with potential environmental and health concerns.
Accurate and sensitive detection methods for CHL are crucial for monitoring its presence in various matrices.
Existing detection methods may lack sensitivity or require complex sample preparation.

Purpose of the Study:

To develop a novel, sensitive, and selective sensor for the detection of chlorothalonil (CHL).
To utilize the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and ratiometric fluorescent quantum dots (RF-QDs) for CHL sensing.
To investigate the mechanism of CHL inhibition on papain (PAP) activity for signal transduction.

Main Methods:

Fabrication of RF-QDs using two different color CdTe QDs.
Utilizing the IFE between AuNPs and RF-QDs for fluorescence quenching.
Employing protamine (PRO) for fluorescence restoration and papain (PAP) for signal modulation.
Assessing CHL's inhibitory effect on PAP activity to quantify CHL concentration.
Characterizing structural changes in PAP to understand CHL inhibition mechanism.

Main Results:

The developed sensor demonstrated a linear response to CHL in the range of 0.34-2320 ng/mL.
A low detection limit of 0.0017 ng/mL for CHL was achieved under optimal conditions.
The sensor exhibited good selectivity for CHL based on its inhibitory effect on PAP activity.
Successful detection of CHL in food and environmental samples confirmed the practical applicability of the sensor.

Conclusions:

A sensitive and selective sensor for CHL detection was successfully developed based on IFE and enzyme inhibition.
The proposed method offers a promising approach for monitoring CHL in real-world samples.
The study provides insights into the mechanism of CHL's inhibitory action on PAP activity.