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Updated: Dec 28, 2025
Detection of Lung Tumor Progression in Mice by Ultrasound Imaging
Published on: February 27, 2020
SP70-Targeted Imaging for the Early Detection of Lung Adenocarcinoma
Jian Xu1, Shichang Zhang1, Wei Zhang1
1Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China.
Abstract:
NJ001 is a monoclonal antibody that can specifically recognize the SP70 antigen on lung adenocarcinoma cells. The goal of this study was to explore its utility in targeted imaging. Subcutaneous xenograft and orthotopic lung tumor implantation BALB/c mouse models were established. Near-infrared fluorescent CF750-labeled NJ001 was injected into two tumor mouse models. Mice that received orthotopic lung tumor implantation were also injected with NJ001-conjugated nanomagnetic beads intravenously, and then underwent micro-CT scanning. Meanwhile, mice with lung tumor were intravenously injected with normal saline and bare nanomagnetic beads as a control. Fluorescence could be monitored in the mice detected by anti-SP70 fluorescence imaging, which was consistent with tumor burden. Signal intensities detected with SP70-targeted micro-CT scans were greater than those in control mice. More importantly, orthotopic tumor lesions could be found on the fourth week with SP70-targeted imaging, which was 2 weeks earlier than detection in the control. Our results suggest that SP70 is a promising target for molecular imaging, and molecularly targeted imaging with an NJ001-labeled probe could be applied for the early detection of lung adenocarcinoma.
Insights
SP70 is a promising target for early lung adenocarcinoma detection. NJ001-labeled molecularly targeted imaging offers improved visualization and earlier diagnosis compared to conventional methods.
Area of Science:
- Oncology
- Medical Imaging
- Immunology
Background:
- Lung adenocarcinoma is a significant global health concern.
- Early detection remains a critical challenge for improving patient outcomes.
- SP70 antigen is expressed on lung adenocarcinoma cells, presenting a potential diagnostic target.
Purpose of the Study:
- To evaluate the utility of NJ001 monoclonal antibody for targeted molecular imaging of lung adenocarcinoma.
- To assess the efficacy of SP70-targeted imaging for early tumor detection in preclinical models.
Main Methods:
- Development of mouse models for subcutaneous xenograft and orthotopic lung tumor implantation.
- Utilized near-infrared fluorescent CF750-labeled NJ001 for fluorescence imaging.
- Employed NJ001-conjugated nanomagnetic beads with micro-CT scanning for enhanced imaging.
- Compared SP70-targeted imaging with control groups receiving saline and bare nanomagnetic beads.
Main Results:
- Fluorescence imaging correlated with tumor burden, confirming SP70 antigen detection.
- SP70-targeted micro-CT scans showed significantly higher signal intensities than control scans.
- Orthotopic lung tumors were detected 2 weeks earlier using SP70-targeted imaging compared to controls.
Conclusions:
- SP70 serves as a viable and promising target for molecular imaging in lung adenocarcinoma.
- NJ001-based molecularly targeted imaging facilitates earlier detection of lung adenocarcinoma.
- This approach holds potential for improving diagnostic capabilities and patient management.
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