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Updated: Dec 28, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
High level of circulating microRNA-142 is associated with acute myocardial infarction and reduced survival
Xian Guo1,2, Yu Chen3, Yang Lu1
1Department of Cardiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, Yangpu District, China.
Background:
Recent study reported that microRNA-142 (miR-142) were up-regulated in the atherosclerotic plaques, which may be responsible for pathogenesis of atherosclerosis. However, whether it associates with presence of acute myocardial infarction (AMI), and its prognostic value is still unknown. We, therefore, investigated the association between miR-142 expression and presence of AMI, and its prognostic value in AMI patients.
Methods:
We included 300 AMI patients and 100 subjects as the control group. MiR-142 content was detected by quantitative real-time polymerase chain reaction. MiR-142 level was identified in all subjects. The multivariate logistic regression analysis were performed to evaluate the risk factors of AMI. The Kaplan-Meier analysis was performed to determine the major adverse cardiovascular and cerebrovascular events (MACCE)-free survival.
Results:
AMI group had significantly higher miR-142 level in comparison to the controls [4.10 (2.03-7.43) vs. 1.92 (0.91-2.91), p < 0.001], moreover, miR-142 content was significantly associated with cardiac troponin I (cTnI) level (r = 0.707, p < 0.001). The MACCE-free survival was significantly lower over 24-month for patients in miR-142 high expression group (72.4% ± 5.6% vs. 76.4% ± 5.1%) (p = 0.022). After adjusting for the traditional risk factors, the odds ratios of miR-142 was 14.74 (95% CI, 2.15-101.24). The multivariate logistic regression analysis revealed that miR-142 level significantly associated with presence of AMI (p < 0.001).
Conclusion:
The serum level of miR-142 was increased in AMI patients when compared with health population. Furthermore, use of this marker may allow a certain predictor of the MACCE in AMI patients.
Insights
Serum microRNA-142 (miR-142) levels are elevated in acute myocardial infarction (AMI) patients. Higher miR-142 expression predicts major adverse cardiovascular and cerebrovascular events (MACCE) in AMI patients.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biomarker Discovery
Background:
- MicroRNA-142 (miR-142) is implicated in atherosclerosis pathogenesis.
- The association of miR-142 with acute myocardial infarction (AMI) and its prognostic value remain unclear.
Purpose of the Study:
- To investigate the association between miR-142 expression and the presence of AMI.
- To evaluate the prognostic value of miR-142 in AMI patients.
Main Methods:
- Quantitative real-time polymerase chain reaction was used to measure miR-142 levels in 300 AMI patients and 100 controls.
- Multivariate logistic regression and Kaplan-Meier analysis were employed to assess risk factors and survival outcomes.
Main Results:
- AMI patients exhibited significantly higher miR-142 levels compared to controls (p < 0.001).
- miR-142 levels correlated positively with cardiac troponin I (cTnI) (r=0.707, p < 0.001).
- High miR-142 expression was associated with reduced 24-month MACCE-free survival (p=0.022) and independently predicted AMI presence (OR=14.74).
Conclusions:
- Serum miR-142 levels are elevated in AMI patients.
- miR-142 serves as a potential predictor for MACCE in patients with AMI.
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