Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modeling Glioma Stem Cell-Mediated Tumorigenesis Using Zebrafish Patient-Derived Xenograft Systems.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Targeting Hsp70 Immunosuppressive Signaling Axis with Lipid Nanovesicles: A Novel Approach to Treat Pancreatic Cancer.

Cancers·2025
Same author

Communicating Adolescent and Young Adult Oncology Treatment Guidelines in Practice: The Importance of Documentation for Ensuring Positive Impacts on Oncology Care.

Journal of adolescent and young adult oncology·2024
Same author

Machine learning-driven identification of the gene-expression signature associated with a persistent multiple organ dysfunction trajectory in critical illness.

EBioMedicine·2023
Same author

Obesity Alters cytokine signaling and gut microbiome in septic mice.

Innate immunity·2023
Same author

Olfactomedin-4 <sup>+</sup> neutrophils exacerbate intestinal epithelial damage and worsen host survival after <i>Clostridioides difficile</i> infection.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Dec 28, 2025

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
14:54

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Published on: January 27, 2019

11.7K

Juvenile OLFM4-null mice are protected from sepsis.

Julie E Stark1, Amy M Opoka1, Jaya Mallela1

  • 1Department of Pediatrics, University of Cincinnati College of Medicine, and Division of Critical Care Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.

American Journal of Physiology. Renal Physiology
|February 19, 2020
PubMed
Summary

Pediatric sepsis and acute kidney injury (AKI) mechanisms are unclear. This study shows that removing Olfactomedin-4 (OLFM4) improves survival in septic mice, suggesting a kidney-specific role for OLFM4 in sepsis.

Keywords:
infectionkidney injuryneutrophilolfactomedin-4pediatric

More Related Videos

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
06:02

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

Published on: November 6, 2020

17.4K
Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

1.3K

Related Experiment Videos

Last Updated: Dec 28, 2025

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
14:54

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Published on: January 27, 2019

11.7K
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
06:02

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

Published on: November 6, 2020

17.4K
Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

1.3K

Area of Science:

  • Pediatric critical care medicine
  • Nephrology
  • Immunology

Background:

  • Pediatric sepsis is a major cause of child mortality.
  • Sepsis-associated acute kidney injury (AKI) is common but poorly understood.
  • Olfactomedin-4 (OLFM4) is linked to poor sepsis outcomes.

Purpose of the Study:

  • Investigate the role of OLFM4 in pediatric sepsis-induced AKI.
  • Elucidate the mechanisms of OLFM4 in sepsis.
  • Determine if OLFM4 contributes to kidney injury during sepsis.

Main Methods:

  • Utilized a pediatric murine model of sepsis.
  • Compared survival rates between wild-type and OLFM4-null mice.
  • Performed immunohistochemistry to assess OLFM4 expression in kidneys.
  • Measured renal cell apoptosis and plasma creatinine levels.
  • Conducted bone marrow transplant experiments.

Main Results:

  • OLFM4-null mice showed significantly increased survival after sepsis induction.
  • Increased OLFM4 expression was observed in the kidney's loop of Henle in septic wild-type mice.
  • Renal cell apoptosis and plasma creatinine were elevated in wild-type compared to OLFM4-null mice.
  • Bone marrow transplant indicated local kidney production of OLFM4.

Conclusions:

  • Renal expression of OLFM4 is demonstrated for the first time.
  • OLFM4 may play a kidney-specific role in sepsis pathogenesis.
  • Targeting OLFM4 could offer a therapeutic strategy for pediatric sepsis-AKI.